Table 2.
List of strains and plasmids
| Strain/plasmid name | Description | Source |
|---|---|---|
| Strain | ||
| E. coli E69 | Serotype O9a:K30 | F. Orskov |
| K. pneumoniae 264(1) | Serotype O7:K67 | F. Orskov |
| CWG28 | E. coli E69 derivative; trp his lac rpsL cpsK30; Smr | Ref. 52 |
| CWG634 | CWG28 derivative; manA; Smr, Tcr | Ref. 17 |
| CWG635 | CWG634 derivative; wbdD::aacC1; Smr, Tcr, Gmr | Ref. 17 |
| CWG638 | CWG634 derivative; Δwzm-wzt::aphA-3; Smr Tcr Kmr | Ref. 17 |
| CWG900 | CWG634 derivative; ΔwbdD; Smr, Tcr | Ref. 16 |
| CWG1412 | CWG635 derivative; Δwzm-wzt::aphA-3; Smr Tcr Kmr, Gmr | This study |
| Plasmid | ||
| pBAD24 | Plasmid vector with l-arabinose-inducible promoter; Apr | Ref. 53 |
| pKD3 | Source of chloramphenicol resistance cassette; Cmr | Ref. 54 |
| pWQ470 | pBAD24 derivative containing His6-wbdD | Ref. 16 |
| pWQ829 | pWQ470 derivative containing His6-wbdDH132A | Ref. 19 |
| pWQ830 | pWQ470 derivative containing His6-wbdDD351A | Ref. 19 |
| pWQ831 | pWQ470 derivative containing His6-wbdDY16FA | Ref. 19 |
| pWQ391 | pBAD24 derivative containing a fragment including wzm-wzt from K. pneumoniae O2a and the Cmr cassette from pKD3 cloned into the ScaI site; Cmr | Ref. 11 |
| pWQ1013 | pBAD24 derivative containing the open-reading frame encoding His6-Wzt-C (amino acids 268–464) from K. pneumoniae O7 cloned between the NheI and NcoI sites | This study |
| pWQ1014 | pBAD24 derivative containing the open reading frame encoding Wzm-Wzt-His6 tag from K. pneumoniae O7 cloned between the NheI and NcoI sites | This study |
| pWQ332 | pBAD24 derivative containing E. coli O9a Wzm-Wzt-His6 | Ref. 22 |
| pWQ424 | pBAD24 derivative containing E. coli O9a Wzt-C-His10 | Ref. 23 |