Figure 2.

Histological analysis of peripheral organs involved in iron homeostasis reveals inflammation and necrosis in the liver. A, representative H&E analysis of liver at ×5 (scale bars = 50 μm) and spleen and duodenum at ×20 (scale bars = 200 μm) from Fpn^fl/fl and Fpn^ΔIE cohorts. B, representative H&E and picrosirius red analysis of liver from Fpn^fl/fl and Fpn^ΔIE cohorts; images ×20, scale bars = 200 μm. C, additional representative H&E images of liver from Fpn^ΔIE cohorts; images at ×20, scale bars = 200 μm. D, qPCR analysis of inflammatory genes in livers of Fpn^fl/fl and Fpn^ΔIE cohorts. All data are from mice 6 months post-tamoxifen treatment. Mean ± S.E. are plotted. Significance was determined using two-tailed unpaired t test. *, p < 0.5 compared between Fpn^fl/fl and Fpn^ΔIE cohorts.