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. 2019 Aug 20;294(41):15052–15067. doi: 10.1074/jbc.RA119.007404

Figure 2.

Figure 2.

Overexpression of miR-145 effectively induced EMT in HMrSV5 cells. A, HMrSV5 cells were transfected with miR-145 mimics or ctrl mimics for 48 h. Then cells were harvested. The miR-145 expression level was analyzed by RT-qPCR. B–D, the protein expression levels of FN1, Col1α1, and α-SMA (B) and E-cad (D) were evaluated by Western blotting. The mRNA level of FN1 and α-SMA was evaluated by RT-qPCR (C). E, HMrSV5 cells were transfected with miR-145 inhibitor or ctrl inhibitor for 48 h before cells were harvested. The miR-145 expression level was analyzed by RT-qPCR. F–H, the protein expression levels of FN1, Col1α1, and α-SMA (F) and E-cad (H) were examined by Western blotting. The mRNA level of FN1 and α-SMA was evaluated by RT-qPCR (G). Data are presented as the mean ± S.D. (error bars) for three independent samples in each group. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus the control group.