Knockdown of FGF10 induced the procession of EMT in HMrSV5 cells.
A and B, HMrSV5 cells were transfected with negative control siRNA or si-FGF10. After 48 h, cells were harvested, and the transfection efficacy was analyzed by RT-qPCR (A) and Western blotting (B). C and D, HMrSV5 cells were transfected with negative control siRNA or si-FGF10. After 48 h, cells were harvested. Western blot analysis was used to detect the protein levels of FN1, Col1α1, and α-SMA (C) and E-cad (D). Data are presented as the mean ± S.D. (error bars). *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus the NC group.