Figure 5.

miR-145 targeted the 3′-UTR of FGF10. A, using the TargetScan database, the nucleic acid sequence of miR-145-5p was predicted to contain binding sites for the 3′-UTR of FGF10 in different species. B and C, HMrSV5 cells were transfected with miR-145 mimics or ctrl mimics for 48 h. The mRNA (B) and protein levels (C) of FGF10 were analyzed by RT-qPCR and Western blotting. D, luciferase activity of HMrSV5 cells transfected with a luciferase reporter containing the WT or mutated 3′-UTR of FGF10. Then cells were cotransfected with miR-145 mimics or ctrl mimics, and the normalized levels of luciferase activity are shown. The results are presented as the mean ± S.D. (error bars) from three independent experiments. The -fold change is statistically significant. *, p < 0.05; **, p < 0.01; ***, p < 0.001.