Figure 6.

miR-145 promoted EMT and peritoneal fibrosis by targeting FGF10 in HMrSV5 cells. A and B, HMrSV5 cells were transfected with miR-145 inhibitor. After 48 h, cells were harvested. The mRNA levels (A) and protein levels (B) of FGF10 were determined by RT-qPCR and Western blotting. C and D, HMrSV5 cells were transfected with si-FGF10 for 48 h before a second transfection of miR-145 mimics or an inhibitor for 48 h. Then cells were harvested, and the protein levels of FN1, Col1α1, α-SMA (C), and E-cad (D) were analyzed by Western blotting. The results are presented as the mean ± S.D. (error bars) from three independent experiments. The -fold change is statistically significant. *, p < 0.05; **, p < 0.01; ***, p < 0.001.