Figure 9.

Overexpression of miR-145 aggravated peritoneal fibrosis in vivo, as determined by RT-qPCR, Western blotting, and immunohistochemistry. A and B, immunohistochemistry was applied to assess the expression of Col1α1 (A) and FGF10 (B) in the anterior abdominal walls. C and D, the mRNA levels of FGF10 (C) and FN1 and α-SMA (D) were determined by RT-qPCR. E and F, Western blotting confirmed the expression of FN1, α-SMA, and Col1α1 (E) and E-cad (F). The results are presented as the mean ± S.D. (error bars) from three independent experiments. The -fold change is statistically significant. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Scale bar, 50 μm.