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. 2019 Sep 6;294(41):14860–14875. doi: 10.1074/jbc.RA119.008374

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© 2019 Masuda et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Analysis of the effects of different E2s and their concentrations. A–E, titration of E2s. The indicated E2s and mutants were used in the standard reactions. The reaction products were visualized by Western blotting with the indicated antibodies. F, E2-charging assay. E2-charging assays were performed as described under “Experimental procedures,” and the reaction products were analyzed under nonreducing or reducing conditions by Western blotting with anti-UBCH5c. G, E3-charging assay. E3-charging assays were performed in the absence of E6, and the reaction products were analyzed under nonreducing or reducing conditions by Western blotting with anti-E6AP. his-tag, ^hisUBCH5c.