Figure 5.

HPAEC-PAD profiles of soluble reaction products generated by C4-oxidizing NcLPMOs from KGM (A) and KGM coated on PASC (B). Reaction mixtures contained 1 μm NcLPMO9A (black), 1 μm NcLPMO9C (red), or 1 μm NcLPMO9D (blue) and 2 mg·ml⁻¹ KGM (A) or 2 mg·ml⁻¹ KGM and 2 mg·ml⁻¹ PASC (B) with (solid lines) or without (dashed lines) AscA. Note that the LPMO activity not only leads to generation of KGM-derived products eluting between 24 and 40 min but also to a shift of the broad substrate peak that is visible in the control reactions without AscA. Peaks eluting prior to 24 min include oxidized AscA and, in B, oxidized cello-oligomers.