Hsp70.1/.3−/− mice have decreased numbers of eosinophils and reduced levels of Th2 cytokines in the BAL fluid. BAL fluid was collected from WT (filled circles and squares) and Hsp70.1/.3−/− (open circles and squares) mice challenged with saline or SEA. A, the total BAL cell number was determined. B, the differential cell number in the BAL fluid was determined with Cytospin preparations. C–E, levels of Th2 cytokines IL-4 (C), IL-5 (D), and IL-13 (E) were measured by ELISA. The data are shown as the mean ± S.D. (error bars), n = 5–6 per group. Significant p values are shown as *, **, ***, and **** for p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively. Except for A and B shown as pooled data from two different experiments with similar results (n = 10–12 mice per group), data are shown as a representative of two different experiments with similar results (n = 5–6 mice/group).