Table 5. Buy-in and per sample costs among methods.

Costs associated with the implementation of the different methods. In segment (A) we present buy-in costs of oligos and iTru primers and costs per sample of library prep which consists of both, fixed and variable costs depending on pooling at early stages. Segment (B) is the cost of library prep (not considering primers/adapters) per sample given a number of samples. Segment (C) is the total experimental cost of primers/adapters and library prep according to the number of samples in the experiment, the first section is in terms of number of samples, the second section is in terms of plates, each plate consisting of 96 samples. Costs for iTru are calculated using list prices of aliquots from baddna.uga.edu. Costs for ‘oligos’ are calculated using list prices from Integrated DNA Technologies (IDT; Coralville, IA). Other costs are from listed prices from various vendors in January 2019. Please view Files S1 and S6 for additional details on price calculations and also to review total prices of experiment given a number of samples.

(A)		Method 1	Method 2	Method 3	Method 4	Method 5
iTru buy-in		$500	$500	$500	$500	$500
Oligo buy-in		$103	$460	$290	$40	$445
Library Cost per sample		$18.86	variable	variable	$4.44	variable
Fixed cost		$18.86	$3.12	$1.39	$4.44	$1.39
Variable cost		–	$4.07	$17.52	–	$4.07