Figure 5. Agarose gel with 3RAD, 2RAD, and ddRAD library products performed on pUC19 vector with an input quantity of 0.5 ng.

The band close to the 200 bp size standard (arrow above) is that corresponding to a proper library construct. The band below the 100 pb size standard (arrow below) corresponds to adapter-dimers (File S5). The gel indicates that 3RAD libraries outperformed the other two types of libraries tested by decreasing the adapter-dimers and therefore increasing the quantity of desired library constructs.