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. 2019 Oct 8;10(56):5745–5754. doi: 10.18632/oncotarget.27196

Figure 4. USP5 regulates apoptotic responsiveness to TRAIL.

Figure 4

(A) A375 melanoma cells (TRAIL sensitive) were infected with control and USP5 shRNA lentiviral vectors, selected in puromycin and treated with TRAIL 100 ng for indicated hr. Cell lysates were immunoblotted for PARP, USP5, p53, BAX protein indicated and annexin V assessment for cell death (%). (B) SK-Mel28 (p53 mutant) melanoma cells were infected with control and USP5 shRNA lentiviral vectors, selected in puromycin and treated with TRAIL 100 ng for 4 hr. (C) Cell lysates were immunoblotted for the protein indicated and annexin V assessment for cell death (right). (D) Pancreatic (Panc1) cells (TRAIL resistant) were infected with control and USP5 shRNA lentiviral vectors, selected in puromycin and treated with TRAIL 100 ng for 6 hr. Cell lysates were subjected to immunoblotting for the protein indicated and annexin V assessment for cell death (%). (E) U87MG glioblastoma cells (TRAIL resistant) were infected with control and USP5 shRNA lentiviral vectors, selected in puromycin and treated with TRAIL 100 ng for 6 hr. Cell lysates were immunoblotted for the protein indicated and annexin V assessment for cell death (%). Actin served as a loading control.