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. 2019 Oct 8;10(56):5824–5834. doi: 10.18632/oncotarget.27217

Figure 4. HDAC inhibition rescues Hsp90 acetylation in TSC1-knock out cells.

Figure 4

(A) Endogenous Hsp90 was immunoprecipitated from HEK293 cells treated with or without 1 μM pan-HDAC inhibitor ACY-241 for 16 hr and its acetylation status was determined by immunoblot. (B) Hsp90α-WT-FLAG and non-acetylatable mutant K407/K419A were transiently expressed in HEK293 cells in the presence or absence of 1 μM ACY-241 for 16 hr. Acetylation status was evaluated by immunoprecipitation followed by immunoblot. LE=long exposure, SE=short exposure. (C) TSC1 WT HAP1 cells and TSC1 KO HAP1 cells were treated with or without 1μM ACY-241 for 16 hr. Endogenous Hsp90 was isolated and its acetylation status was examined by immunoblot.