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. 2019 Jul 19;8(11):e1641391. doi: 10.1080/2162402X.2019.1641391

Figure 5.

Figure 5.

Functional role of tumor-associated NK cells in sepsis-induced tumor inhibition. Mice were subjected to s.c. inoculation of MCA205 tumor cells followed followed 14 days later by a septic insult (sham surgery or CLP, or PBS or LPS i.p. challenge). (a) Tumors were removed at 24 hours following the septic challenge for the assessment of intracellular expression of IFNγ, perforin, granzyme B and outer membrane expression of CD107a among tumor-associated NK cells. 6–11 mice per group from two independent experiments. *p < .05. (b) Mice were NK-depleted by i.p. injection of anti-NK1.1 antibody 24h before sham surgery (n = 9) or CLP (n = 12). Injection of anti-NK1.1 antibody was repeated once three days later. Tumor volumes were measured before (D14) and 7 days after surgery (D14 + 7). (c) Mice were NK-depleted by i.p. injection of anti-NK1.1 antibody 24h before PBS (n = 11) or LPS (n = 11) i.p. challenge. Injection of anti-NK1.1 antibody was repeated once three days later. Tumor volumes were measured before (D14) and 7 days after surgery (D14 + 7).