. 2019 Jul 3;8(10):e1629780. doi: 10.1080/2162402X.2019.1629780

Table 1.

Neoantigens sequences of the identified mutated genes and their binding affinity with HLA molecules as described in material and methods. Specific mutations induce new antigen proteins which are recognized for peptides of HLA isoforms. Affinity is shown with % Rank.

	Gene	Mutation	Original sequence	Mutated sequence	Recognition peptide	nM	Rank
HLA-A0101	*FUK*	M318I	QPLTMAYVS	QPLTIAYVS	ATQPLTIAY	43.9	0.08
	*SNAPC3*	T129S	TDLVTLGVR	TDLVSLGVR	DTDLVSLGV	161.2	0.2
	*LCTL*	R347W	HFTTRYITE	HFTTWYITE	GTGHFTTWY	239.8	0.25
	*TDO2*	T30A	DKSQTGVNR	DKSQAGVNR	ATDKSQAGV	456.7	0.4
HLA-A0201	*SNAPC3*	T129S	TDLVTLGVR	TDLVSLGVR	YLDTDLVSL	5	0.03
	GC	T347A	MDKYTFELS	MDKYAFELS	LMDKYAFEL	6.6	0.06
	*DOT1L*	V240M	SVIFVNNFA	SVIFMNNFA	FMNNFANFA	9.7	0.125
	*CPSF1*	S356T	DGMRSVRAF	DGMRTVRAF	ALADGMRTV	16.3	0.25
HLA-A0301	*ANO8*	E116Q	TATYESLLR	TATYQSLLR	ATYQSLLRK	11.9	0.025
	*OGN*	G280A	PIVLGKHPN	PIVLAKHPN	SAYPIVLAK	27.1	0.125
	*FBXW10*	R532T	LKTFRHKDP	LKTFTHKDP	KTFTHKDPK	54.2	0.25
HLA-A2402	*DOT1L*	V240M	SVIFVNNFA	SVIFMNNFA	IFMNNFANF	15.1	0.015
	*HIST1H3F*	S58L	RYQKSTELL	RYQKLTELL	RYQKLTELL	18.9	0.02
	*LCTL*	R347W	HFTTRYITE	HFTTWYITE	WYITEWSLL	58.7	0.125
HLA-A2601	*MYBPH*	I230V	DQDSILFIR	DQDSVLFIR	EIPDSVIFM	24.6	0.04
	*STX2*	I232M	MINNIERNV	MINNMERNV	EVKKMINNM	48.1	0.07
	*VNN2*	D81N	VTPEDALYG	VTPENALYG	DVTPENALY	88.6	0.1
	*ANO8*	V759L	FGYVVLFSS	FGYVLLFSS	EMFGYVLLF	329.9	0.25
	*ANO8*	R105C	HRHTRAYAF	HRHTCAYAF	CAYAFTATY	347.7	0.25
	*GP1BA*	S601W	FLRGSLPTF	FLRGWLPTF	FLRGWLPTF	670.7	0.4
HLA-B0702	*FRMD6*	H418P	SSAIHRKLK	SSAIPRKLK	IPRKLKLEL	4.8	0.01
	*CPSF1*	G923R	EEGAGARGR	EEGARARGR	GARARGRAL	10.6	0.04
	*CPSF1*	G923R	EEGAGARGR	EEGARARGR	RARGRALRL	19.1	0.09
	*GHRL*	E66K	GGQAEGAED	GGQAKGAED	SPRGGGQAK	36.6	0.175
	*GTPBP3*	R323W	VRRARERLE	VRRAWERLE	RAREVRRAW	40.9	0.2
HLA-B0801	*SMTN*	M703T	SGSTMMQTK	SGSTTMQTK	TMQTKRHEL	23.4	0.07
	*CNPY3*	G223R	AALGGKKSK	AALGRKKSK	LGRKKSKAA	25.3	0.07
	*CPSF1*	G923R	EEGAGARGR	EEGARARGR	GARARGRAL	69.1	0.2
	*CPSF1*	G923R	EEGAGARGR	EEGARARGR	RARGRALRL	123.1	0.4
	*KLC1*	E325Q	KRALEIREK	KRALQIREK	QIREKKQGL	172.9	0.4
HLA-B1501	*CPSF1*	P262H	LTSLPFDCT	LTSLHFDCT	SLHFDCTPF	20.2	0.175
HLA-B1501	*FBXW10*	Q788E	KAQKQGQLE	KAQKEGQLE	AQKEGQLEF	24.1	0.25
HLA-B2705	*ANO8*	R105C	HRHTRAYAF	HRHTCAYAF	HRHTCAYAF	18	0.06
	*CPSF1*	H964P	ALRLHPMAI	ALRLPPMAI	GRALRLPPM	30.7	0.125
	*HIST1H3F*	S58L	RYQKSTELL	RYQKLTELL	LRYQKLTEL	59.5	0.25
	*FBXW10*	T983A	FRVNTEFVL	FRVNAEFVL	FRVNAEFVL	61.7	0.3
HLA-B3901	*CPSF1*	T266P	PFDCTQALA	PFDCPQALA	TPFDCPQAL	10.2	0.025
	*ANO8*	R105C	HRHTRAYAF	HRHTCAYAF	HRHTCAYAF	13.5	0.04
	*FBXW10*	T983A	FRVNTEFVL	FRVNAEFVL	FRVNAEFVL	16.9	0.05
	*RDX*	E499Q	EASAELSNE	EASAQLSNE	AQLSNEAAL	93	0.175
	*OGN*	D112H	EVDIDAVPP	EVDIHAVPP	IHAVPPPPL	80.8	0.175
	*ANO8*	V759L	FGYVVLFSS	FGYVLLFSS	LKEMFGYVL	116.5	0.2
	*FUK*	S56R	KRVGSGGAT	KRVGRGGAT	GRGGATQPL	195	0.3
	*ATAD3A*	M432I	DVAPMGREG	DVAPIGREG	SKAADVAPI	189.4	0.3
	*KLC1*	Y313H	KRGKYKEAE	KRGKHKEAE	HKEAEKRAL	194.1	0.3
	*RAB10*	F10L	YDLLFKLLL	YDLLLKLLL	ERLEYDLLL	267.8	0.4
	*LCTL*	D459H	WSLLDKFEW	WSLLHKFEW	HKFEWAGGI	246.9	0.4
	*SNAPC3*	T129S	TDLVTLGVR	TDLVSLGVR	YLDTDLVSL	268.9	0.4
HLA-B4001	*ANO8*	V759L	FGYVVLFSS	FGYVLLFSS	KEMFGYVLL	4.3	0.01
	*OGN*	K119T	PPLPKESAY	PPLPKESAY	KESAYPIVL	6.2	0.015
	*DOT1L*	E46G	RWVCEEIPD	RWVCGEIPD	GEIPDSVIF	11.2	0.05
	*RAB10*	F10L	YDLLFKLLL	YDLLLKLLL	LEYDLLLKL	17.2	0.08
	*FBXW10*	T983A	FRVNTEFVL	FRVNAEFVL	LEFRVNAEF	90.6	0.3
	*UBA5*	R17Q	QELERELAQ	QELEQELAQ	LEQELEQEL	126.1	0.4
	*GTPBP3*	R323W	VRRARERLE	VRRAWERLE	WERLEYDLL	104	0.4
	*SNAPC3*	G178E	MIEEGELIL	MIEEEELIL	IEEEELILM	115.3	0.4
HLA-B5801	*LCTL*	R347W	HFTTRYITE	HFTTWYITE	FTTWYITEW	5.3	0.025
		D459H	WSLLDKFEW	WSLLHKFEW	WSLLHKFEW	16	0.1
		R347W	HFTTRYITE	HFTTWYITE	RGTGHFTTW	34.2	0.175
	*EHMT2*	A635T	ALEKALVIQ	ALEKTLVIQ	KALEKTLVI	40.7	0.2
	*ANO8*	R105C	HRHTRAYAF	HRHTCAYAF	CAYAFTATY	44.9	0.25
	*RAB10*	F10L	YDLLFKLLL	YDLLLKLLL	LLLKLLLRW	42.3	0.25
	*EHMT2*	A635T	ALEKALVIQ	ALEKTLVIQ	KTLVIQLNL	64.6	0.3