Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 16;8(10):e1625687. doi: 10.1080/2162402X.2019.1625687

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Taylor & Francis Group, LLC

PMC Copyright notice

Figure 2. — Mice with complete response (CR) were protected from tumor re-establishment in a T cell-dependent manner.

a) Mice with complete response (CR) and naïve () mice were challenged with 1 × 10⁶ C26 tumor cells by SC injection into the right flank (n = 10/group). Mice with CR underwent prior depletion of CD4 (), CD8 (), CD4/8 () or control IgG injections (), (150 µg anti-CD8/4, 300 µg IgG, day −1, 3, 5, 12 and 19) by IP administration. Kaplan-Meier plot is shown of a representative experiment (of 2 with similar results) and a Log-rank test indicated significant differences (p < .0001: naïve vs CR+IgG, naïve vs CR+aCD4, naïve vs CR+aCD8, CR+IgG vs CR+aCD4/8, CR+CD8 vs CR+CD4/8; p < .001: CR+CD4 vs CR+CD4/8; p < .01: naïve vs CR+aCD4, CR+IgG vs CR+aCD4). b) C26 tumor cells (1x10⁶) were inoculated IV into mice with CRs (solid line) and naïve mice (dashed line, n = 10/group) to produce lung metastases. Kaplan-Meier plot is shown of a representative experiment (of 3 with similar results) and a log-rank test showed a significant difference (p < .0001) between groups. c) C26 tumor cells (1x10⁶) were inoculated SC into the right flank and 2.5 × 10⁵ C26 tumor cells were inoculated IV 7 days later. An additional 10 mice received IV tumor cells only (dotted line). Vehicle (dashed line) or INT230-6 (solid line) were administered IT into 275mm³ (50µl/400mm³ C26 tumor) SC tumors (n = 10/group) for 5 sequential days (day 0 to 4) starting on day 14 and tumor growth was monitored. Kaplan-Meier plot of a representative experiment (of 2 with similar results) is shown (top left), and a log-rank test indicated significant differences (p < .01: C26 iv only vs vehicle; p < .0001: C26 iv only vs INT230-6, vehicle vs INT230-6). Quantification of lung nodules at the time of death (n = 9–10/group) is presented (bottom left). Median test was applied for vehicle vs INT230-6 treatment groups. Representative lungs that had been injected with 15% black India ink are shown on the right. Healthy lung tissue is black and smooth whereas tumor nodules appear white and nodular.

Inline graphic — Mice with complete response (CR) were protected from tumor re-establishment in a T cell-dependent manner.

a) Mice with complete response (CR) and naïve () mice were challenged with 1 × 10⁶ C26 tumor cells by SC injection into the right flank (n = 10/group). Mice with CR underwent prior depletion of CD4 (), CD8 (), CD4/8 () or control IgG injections (), (150 µg anti-CD8/4, 300 µg IgG, day −1, 3, 5, 12 and 19) by IP administration. Kaplan-Meier plot is shown of a representative experiment (of 2 with similar results) and a Log-rank test indicated significant differences (p < .0001: naïve vs CR+IgG, naïve vs CR+aCD4, naïve vs CR+aCD8, CR+IgG vs CR+aCD4/8, CR+CD8 vs CR+CD4/8; p < .001: CR+CD4 vs CR+CD4/8; p < .01: naïve vs CR+aCD4, CR+IgG vs CR+aCD4). b) C26 tumor cells (1x10⁶) were inoculated IV into mice with CRs (solid line) and naïve mice (dashed line, n = 10/group) to produce lung metastases. Kaplan-Meier plot is shown of a representative experiment (of 3 with similar results) and a log-rank test showed a significant difference (p < .0001) between groups. c) C26 tumor cells (1x10⁶) were inoculated SC into the right flank and 2.5 × 10⁵ C26 tumor cells were inoculated IV 7 days later. An additional 10 mice received IV tumor cells only (dotted line). Vehicle (dashed line) or INT230-6 (solid line) were administered IT into 275mm³ (50µl/400mm³ C26 tumor) SC tumors (n = 10/group) for 5 sequential days (day 0 to 4) starting on day 14 and tumor growth was monitored. Kaplan-Meier plot of a representative experiment (of 2 with similar results) is shown (top left), and a log-rank test indicated significant differences (p < .01: C26 iv only vs vehicle; p < .0001: C26 iv only vs INT230-6, vehicle vs INT230-6). Quantification of lung nodules at the time of death (n = 9–10/group) is presented (bottom left). Median test was applied for vehicle vs INT230-6 treatment groups. Representative lungs that had been injected with 15% black India ink are shown on the right. Healthy lung tissue is black and smooth whereas tumor nodules appear white and nodular.