Figure 2.

Binding of monospecific and bispecific antibodies to TUBO cells. TUBO tumor were cultured in the absence (a, b) or presence (c, d) of 10ng/ml of mouse IFN-γ for 24 h and incubated with different concentrations of FITC-labeled bivalent (homodimer) anti-rErbB2, anti-PD-L1, monovalent anti-rErbB2 (B12xErbB2) or anti-PD-L1 (B12xPD-L1) or bispecific BsPD-L1xrErbB2 antibodies. On the y-axes, the median fluorescence intensity (MFI) FITC is plotted against the antibody concentration in log nM on the x-axes. EC50 in nM for untreated TUBO (B) and IFN-γ treated TUBO (d) tumor cells is shown.