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. 2019 Sep 5;8(11):e1659092. doi: 10.1080/2162402X.2019.1659092

Figure 5.

Figure 5.

JNK-c-Jun activation is involved in PDK1-induced PD-L1 expression in ovarian cancer cells. (a) Immunoblotting of total and phosphorylated forms of JNK and c-Jun in OVCAR3 cells overexpressing PDK1 and SKOV-3 cells depleted of PDK1. (b, c) PD-L1 mRNA and surface expression in PDK1-overexpressing OVCAR3 cells with knockdown of JNK1. (d) PDK1 mRNA expression in OVCAR3 cells transfected with PDK1-expressing plasmid followed by siRNAs targeting JNK1. (e) qPCR assessment of JNK1 mRNA expression. Representative data from three experiments are shown (*P < .05, **P < .01, ****P < .0001).