Dynamic testing. (A) Samples are typically subjected to a specified strain, allowed to relax until equilibrium, and then cyclically compressed at a specified frequency, number of cycles, and strain amplitude. The corresponding stress response is then used to calculate (B) dynamic modulus, the peak-to-trough stress divided by strain. Systematic review of (C) frequency, (D) number of cycles, and (E) strain amplitude show high variability among studies. Green dashed lines represent testing parameters chosen for validation studies. (F) Validation testing of agarose gels determined that dynamic frequency impacts calculated dynamic modulus. Color images are available online.