Figure 6.

Epigenome-wide meta-analysis of associations between urinary arsenic and DNA methylation in Bangladeshi adults using data from the Health Effects of Arsenic Longitudinal Study (HEALS) and the Bangladesh Vitamin E and Selenium Trial (BEST). Using data from EPIC (HEALS; $n=396$) and $450\mathrm{K}$ (BEST; $n=400$) arrays, 390,810 CpG sites were meta-analyzed using METAL [summary statistics from covariate- and surrogate variable (SV)–adjusted model were provided as input]. (A) Q-Q plot of the observed distribution of meta-analysis p-values. The red line represents expected distribution of p-values under the null, and $\mathrm{\lambda }$ corresponds to the genomic inflation factor (see “Methods” section). (B) Manhattan plot of the location of each CpG and p-value for each CpG–arsenic association. Solid and dashed lines designate the Bonferroni threshold ($p=1.3\times {10}^{-7}$) and false discovery rate (FDR) 0.05 threshold ($p=2.8\times {10}^{-5}$), respectively.