Fig 4. Effect of treatment with LDH inhibitor on the Wolbachia-parasite interaction.
A. The wsp copy number per worm in adult males after a 6-day treatment was measured by qPCR (***–p<0.001). B. Relative gene expression analysis of Bm-LDH in samples treated with pyruvate (M+Pyr, grey, triangle), 3BromoPyruvate, inhibitor of glycolysis (M+3BrPyr, Blue, diamond) and control (M, gray, square). The analysis showed that pyruvate treatment induced expression of Bm-LDH after the first day of treatment. Inhibition of glycolysis only activated the expression of Bm-LDH in worms after 3 days of treatment. C. Schematic presentation of the role of Bm-LDH in pyruvate distribution between bacteria and conversion into lactate in parasite cells.