FIG 5.

Acute inhibition of PIKfyve by apilimod alters lysosomal morphology and reduces lysosomal proteolysis in macrophages. (A) Fluorescence microscopy images of bone marrow-derived macrophages treated with apilimod (100 nM) for 2 h and incubated with dextran (green) and DQ BSA (red) over 90 min. (B) Measurement of lysosomal proteolysis normalized to fluid-phase dextran uptake in panel A (n = 3). Statistical analysis was performed using unpaired two-tailed Student’s t test. All error bars indicate SEM. Scale bars: 20 μm.