Table 4.
Evaluation of wrinkles through replica analysis of hairless mouse before autopsy
| NC | VC | E1 | E2 | E3 | ||
|---|---|---|---|---|---|---|
| Wrinkle area (mm2) | 8w | 19.0 ± 10.5** | 50.0 ± 7.5 | 41.5 ± 5.8 | 54.0 ± 31.1 | 57.8 ± 42.1 |
| 12w | 17.8 ± 5.9** | 76.6 ± 26.2 | 19.9 ± 9.9** | 33.2 ± 10.9** | 35.2 ± 43.6* | |
| No. of wrinkles | 8w | 52.0 ± 14.2 | 89.0 ± 40.1 | 95.0 ± 18.4 | 101.5 ± 23.3 | 91.0 ± 7.1 |
| 12w | 46.7 ± 25.4* | 83.0 ± 28.2 | 52.0±2.8* | 68.5 ± 6.4 | 67.5 ± 12.0 | |
| Total length (mm) | 8w | 38.5 ± 3.2** | 97.5 ± 8.0 | 92.6 ± 24.2 | 92.5 ± 29.92 | 81.2 ± 24.1 |
| 12w | 35.5 ± 7.4** | 94.9 ± 37.3 | 30.2 ± 10.3** | 38.6 ± 4.1** | 49.18 ± 8.06* | |
| Mean length (mm) | 8w | 0.6 ± 0.1* | 0.9 ± 0.3 | 1.1 ± 0.2 | 0.9 ± 0.1 | 0.9 ± 0.2 |
| 12w | 0.4 ± 0.1** | 1.1 ± 0.1 | 0.5 ± 0.1** | 0.7 ± 0.2** | 0.8 ± 0.2** | |
| Mean depth (μm) | 8w | 90.6 ± 2.8** | 116.3 ± 13.1 | 103.7 ± 8.6 | 103.6 ± 13.4 | 102.4 ± 7.0 |
| 12w | 84.2 ± 7.1* | 120.7 ± 29.0 | 69.7 ± 14.5* | 90.1 ± 5.1 | 91.7 ± 4.1 |
8w: Just before oral administration, 12w: Four weeks after oral administration. NC: Normal control, VC: Vehicle control, E1: 7-MEGA™ 500 (200 mg/kg), E2: 7-MEGA™ 500 (100 mg/kg), E3: 7-MEGA™ 500 (50mg/kg). Values are means ± SE (n = 7).
*
p < 0.05,
**
p < 0.01 as compared to the VC group by ANOVA and Duncan’s multiple range test.