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. 2019 Sep 10;18(21):2849–2859. doi: 10.1080/15384101.2019.1638190

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — The expression of MALAT1 in OGD/R induced N2A neurons was increased by vitamin B1 and B12. To evaluate the effect of vitB on MALAT1 expression and which levels of vitB were suitable in N2A cells, OGD/R and VitB at different concentrations were introduced to allocate into six groups: control, OGD/R, OGD/R + 1 μM VitB, OGD/R + 2.5 μM VitB, OGD/R + 5 μM VitB, and OGD/R + 7.5 μM VitB. (a) The expression of MALAT1 in N2A cells was detected by qRT-PCR. (b, c) The expression level of miR-1, BDNF mRNA and protein in N2A cells was determined by qRT-PCR and western blot, respectively. *P < 0.05 compared with control; ^#P < 0.05 compared with OGD/R; ^&P < 0.05 compared with 1μM; ^$P < 0.05 compared with 2.5μM.