Figure 5.

FAM83A-AS1 interacts directly with miR-150-5p.

(a) Bioinformatics predicted and mutated miR-150-5p binding sites of FAM83A-AS1. (b) Luciferase activity in HEK293T cells was detected by luciferase reporter assay after cotransfection with pGL3-FAM83A-AS1-WT or pGL3-FAM83A-AS1-MUT and miR-150-5p mimics or miR-NC. (c) The relationship among FAM83A-AS1, miR-150-5p and Ago2 was confirmed by RNA immunoprecipitation (RIP) with an Ago2 antibody or IgG antibody. Real-time PCR was used to detect changes in FAM83A-AS1 or miR-150-5p in precipitates. (d-e) qRT-PCR assays were performed to assess the effect of FAM83A-AS1 on miR-150-5p expression in transfected LAUD cells.