Skip to main content
. 2019 Sep 19;8:e48689. doi: 10.7554/eLife.48689

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Strain, strain background (Mus musculus) Col1a1:tetO-H2B-GFP; ROSA:rtTA (H2B-GFP) Brack, Hochedlinger labs, PMID: 24715455, 19060879
Strain, strain background (Mus musculus) Scx-GFP Schweitzer lab, PMID: 20059955
Strain, strain background (Mus musculus) Scx-Cre Schweitzer lab,PMID: 11585810
Strain, strain background (Mus musculus) Gt(ROSA)26Sortm9(CAG.tdTomato)Hze (ai9)/TdTom Jackson cat# 007909
Antibody Rat anti Mouse CD31 BD Cat#551262 (1:100)
Antibody Rat anti Mouse CD45 BD Cat# 557659 (1:100)
Antibody Mouse monoclonal BrdU Biolegend Cat# 339808 (1:100)
Antibody Rat monoclonal BrdU Abcam Cat# 6326 (1:100)
Commercial assay or kit Green Flow Cytometry Reference Beads Molecular Probes Cat# C16508
Commercial assay or kit Click-iT EdU Invitrogen Cat# C10337
Commercial assay or kit Qubit HS RNA assay Invitrogen Cat# Q32852
Commercial assay or kit SuperScript Thermo Fisher Cat#18091050
Commercial assay or kit SYBR Applied Biosystems Cat#4367659
Chemical compound, drug Dox Sigma cat#D9891
Chemical compound, drug Collagenase II Worthington Cat# LS004176
Chemical compound, drug DMEM Gibco Cat#11956–092
Chemical compound, drug P/S Corning Cat#30002 CL
Chemical compound, drug Hepes Gibco Cat#15630–80
Chemical compound, drug Collagenase I Gibco Cat# 17100–017
Chemical compound, drug Dispase Gibco Cat# 1710541
Chemical compound, drug BrdU Sigma Cat#B5002
Chemical compound, drug Hoechst Thrmo Fisher Cat# H3569
Chemical compound, drug TRIzol Invitrogen Cat# 15596026
Software, algorithm Prism8 GraphPad
Software, algorithm R 'stats' version 3.5.1(Team)