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. 2019 Sep 30;8:e50375. doi: 10.7554/eLife.50375

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© 2019, Genovese et al

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Figure 3. — (A) Clones are labeled with mCherry and observed 8 hours (8h), 2 days (2d), 4 days (4d) and 8 days (8d) after clonal induction (ACI) in poxⁿ > pros^RNAi tumors. mCherry^- tNBs are GFP⁺. Images represent one confocal section. (B) 3D projections of clones 8 hr, 2d, 4d and 8d ACI. The color-code labels clones according to their volume. (C) Three categories of clones can be identified in poxⁿ > pros^RNAi tumors: clones composed of Chinmo⁺Imp⁺ tNBs only (CI clones), clones composed of both Chinmo⁺Imp⁺ and Syp⁺E93⁺ tNBs (MIXED clones), and clones composed of Syp⁺E93⁺ tNBs only (SYP clones). Chinmo⁺Imp⁺ tNBs are identified by the presence of Chinmo. Syp⁺ tNBs are identified by the absence of Chinmo. (D) Proportion of CI (red), MIXED (gray) and SYP (blue) clones 8h, 2d, 4d and 8d ACI. Proportion of CI clones 8h ACI (n = 117 clones from 4 VNCs); 2d ACI (n = 75 clones from 4 VNCs); 4d ACI (n = 13 clones from 5 VNCs); 8d ACI (n = 17 clones from 5 VNCs). P between CI clones at 8h and 2d ACI -> P_CI8h/2d = 0.2; P_CI2d/4d= 0.016; P_CI4d/8d= 0.88. Proportion of MIXED clones 8h ACI (n = 8 clones from 4 VNCs); 2d ACI (n = 28 clones from 4 VNCs); 4d ACI (n = 57 clones from 5 VNCs); 8d ACI (n = 66 clones from 5 VNCs). P_MIXED8h/2d= 0.029; P_MIXED2d/4d= 0.016; P_MIXED4d/8d= 0.31. Proportion of SYP clones 8h ACI (n = 181 clones from 4 VNCs); 2d ACI (n = 196 clones from 4 VNCs); 4d ACI (n = 200 clones from 5 VNCs); 8d ACI (n = 255 clones from 5 VNCs). P_SYP8h/8d= 0.016. Scale bars, 20 µm.

Figure 3—figure supplement 1. — (A) Clones are labeled with mCherry and observed 8 hours (8h), 2 days (2d), 4 days (4d) and 8 days (8d) after clonal induction (ACI) in poxⁿ > pros^RNAi tumors. mCherry^- tNBs are GFP⁺. Images represent one confocal section. (B) 3D projections of clones 8 hr, 2d, 4d and 8d ACI. The color-code labels clones according to their volume. (C) Three categories of clones can be identified in poxⁿ > pros^RNAi tumors: clones composed of Chinmo⁺Imp⁺ tNBs only (CI clones), clones composed of both Chinmo⁺Imp⁺ and Syp⁺E93⁺ tNBs (MIXED clones), and clones composed of Syp⁺E93⁺ tNBs only (SYP clones). Chinmo⁺Imp⁺ tNBs are identified by the presence of Chinmo. Syp⁺ tNBs are identified by the absence of Chinmo. (D) Proportion of CI (red), MIXED (gray) and SYP (blue) clones 8h, 2d, 4d and 8d ACI. Proportion of CI clones 8h ACI (n = 117 clones from 4 VNCs); 2d ACI (n = 75 clones from 4 VNCs); 4d ACI (n = 13 clones from 5 VNCs); 8d ACI (n = 17 clones from 5 VNCs). P between CI clones at 8h and 2d ACI -> P_CI8h/2d = 0.2; P_CI2d/4d= 0.016; P_CI4d/8d= 0.88. Proportion of MIXED clones 8h ACI (n = 8 clones from 4 VNCs); 2d ACI (n = 28 clones from 4 VNCs); 4d ACI (n = 57 clones from 5 VNCs); 8d ACI (n = 66 clones from 5 VNCs). P_MIXED8h/2d= 0.029; P_MIXED2d/4d= 0.016; P_MIXED4d/8d= 0.31. Proportion of SYP clones 8h ACI (n = 181 clones from 4 VNCs); 2d ACI (n = 196 clones from 4 VNCs); 4d ACI (n = 200 clones from 5 VNCs); 8d ACI (n = 255 clones from 5 VNCs). P_SYP8h/8d= 0.016. Scale bars, 20 µm.