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. 2019 Sep 30;8:e50375. doi: 10.7554/eLife.50375

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© 2019, Genovese et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — (A) Cartoon of the stochastic clone model. C stands for Chinmo⁺Imp⁺ tNBs, S stands for Syp⁺E93⁺ tNBs. T_c means ‘division time of Chinmo⁺Imp⁺ tNBs’, T_s means ‘division time of Syp⁺E93⁺ tNBs’. C_q stands for quiescent Chinmo⁺Imp⁺ tNB, S_q stands for quiescent Syp⁺E93⁺ tNB. q_c and q_s are the probabilities for Chinmo⁺Imp⁺ and Syp⁺E93⁺ tNBs to be quiescent after being generated. (B) Dynamics of proportion for each clone category in four extreme model scenarios: No hierarchy, Strict hierarchy #1, Strict hierarchy #2, Plastic hierarchy. Solid lines represent the simulations (n = 1000 clones), dots represent experimental measurements. Cartoons above graphs represent the division probabilities used to generate each graph. (C) Violin plots depicting distributions of clone sizes (number of cells) for each category calculated from the experiments. (D) Violin plots depicting distributions of clone sizes (number of cells) for each category of clones after simulation, using the set of parameters minimizing the error. Center lines of boxes show the medians. (E) Proportion of clones over time in each category using the set of parameters minimizing the error. Solid lines represent the simulations (n = 1000 clones), dots represent experimental measurements. (F) Hierarchical scheme able to recapitulate the dynamics of clone growth and composition, with the parameters measured from the experimental data and defined by the fit. (G) Proportion of clones in each category using the set of parameters minimizing the error while allowing a small chance of reverse division from S to C (top: 1%, bottom: 5%).

Figure 4—figure supplement 1. — (A) Cartoon of the stochastic clone model. C stands for Chinmo⁺Imp⁺ tNBs, S stands for Syp⁺E93⁺ tNBs. T_c means ‘division time of Chinmo⁺Imp⁺ tNBs’, T_s means ‘division time of Syp⁺E93⁺ tNBs’. C_q stands for quiescent Chinmo⁺Imp⁺ tNB, S_q stands for quiescent Syp⁺E93⁺ tNB. q_c and q_s are the probabilities for Chinmo⁺Imp⁺ and Syp⁺E93⁺ tNBs to be quiescent after being generated. (B) Dynamics of proportion for each clone category in four extreme model scenarios: No hierarchy, Strict hierarchy #1, Strict hierarchy #2, Plastic hierarchy. Solid lines represent the simulations (n = 1000 clones), dots represent experimental measurements. Cartoons above graphs represent the division probabilities used to generate each graph. (C) Violin plots depicting distributions of clone sizes (number of cells) for each category calculated from the experiments. (D) Violin plots depicting distributions of clone sizes (number of cells) for each category of clones after simulation, using the set of parameters minimizing the error. Center lines of boxes show the medians. (E) Proportion of clones over time in each category using the set of parameters minimizing the error. Solid lines represent the simulations (n = 1000 clones), dots represent experimental measurements. (F) Hierarchical scheme able to recapitulate the dynamics of clone growth and composition, with the parameters measured from the experimental data and defined by the fit. (G) Proportion of clones in each category using the set of parameters minimizing the error while allowing a small chance of reverse division from S to C (top: 1%, bottom: 5%).