Figure 5. The final proportions of Chinmo⁺Imp⁺ and Syp⁺E93⁺ tNBs are not influenced by their initial proportions and depend on the tumor type.

(A) Proportion of Syp⁺E93⁺ (blue) and Chinmo⁺Imp⁺ (red) tNBs over time using the set of parameter values determined by the error minimization and starting from 100% of Chinmo⁺Imp⁺ tNBs. Dashed lines represent a stochastic simulation (clone model), thin lines represent the prediction of the deterministic model. An equilibrium is rapidly reached were Chinmo⁺Imp⁺ tNBs represent about 20% of all tNBs. (B) Dashed curves: evolution of the proportions of Syp⁺E93⁺ (blue) and Chinmo⁺Imp⁺ (red) tNBs, as predicted by the deterministic model, when starting with 100% of Chinmo⁺Imp⁺. Plain curves: evolution of the proportions of Syp⁺E93⁺ (blue) and Chinmo⁺Imp⁺ (red) tNBs, as predicted by the deterministic model, when starting with 50% of Chinmo⁺Imp⁺ and 50% of Syp⁺E93⁺ tNBs. In both scenarios, the same equilibrium is reached. (C) UAS-snr1^RNAi transgenes are expressed in type II NBs using the ase-Gal80, wor-Gal4, UAS-dicer2 system causing NB tumors that persist in the CB of adult flies. tNBs in 6-day-old adults are marked with Mira, Imp, Chinmo and Syp. (D) Proportion of Chinmo⁺Imp⁺ tNBs in ase-G80, wor > snrRNAi^RNAi-1 (n = 5 CB) and ase-G80, wor > snrRNAi^RNAi-2 (n = 7 CB) tumors in 6-day-old adults (volume of Chinmo+Imp+ tNBs over volume of all tNBs x 100) . p=0.0061. Scale bars, 20 µm.