Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | UAS-Flybow. 1.1 | Bloomington Drosophila Stock Center (Hadjieconomou et al., 2011) |
RRID:BDSC_35537 | |
| Genetic reagent (D. melanogaster) | UAS-mCherrychinmoUTRs | Cédric Maurange (Dillard et al., 2018) |
mCherry reflects the post-transcriptional regulation of chinmo. | |
| Genetic reagent (D. melanogaster) | UAS-SypRNAi1 | VDRC | 33011 | RNAi1 |
| Genetic reagent (D. melanogaster) | UAS-SypRNAi2 | VDRC | 33012 | RNAi2 |
| Genetic reagent (D. melanogaster) | UAS-Syp-RB-HA | Tzumin Lee (Liu et al., 2015) |
||
| Genetic reagent (D. melanogaster) | UAS-prosRNAi1 | Bloomington Drosophila Stock Center | RRID:BDSC_26745 | RNAi1 |
| Genetic reagent (D. melanogaster) | UAS-prosRNAi2 | VDRC | 101477 | RNAi2 |
| Genetic reagent (D. melanogaster) | UAS-ImpRNAi | VDRC | 20322 | |
| Genetic reagent (D. melanogaster) | UAS-chinmoRNAi | Bloomington Drosophila Stock Center | RRID:BDSC_33638 | |
| Genetic reagent (D. melanogaster) | UAS-dicer2 | Bloomington Drosophila Stock Center | RRID:BDSC_24650
RRID:BDSC_24651 |
was used in combination with GAL4 lines in order to improve RNAi efficiency. |
| Genetic reagent (D. melanogaster) | UAS-mCD8::GFP | Bloomington Drosophila Stock Center | RRID:BDSC_5130
RRID:BDSC_32185 |
|
| Genetic reagent (D. melanogaster) | UAS-myr::GFP | Bloomington Drosophila Stock Center |
RRID:BDSC_32197 | |
| Genetic reagent (D. melanogaster) | UAS-mCherry.NLS | Bloomington Drosophila Stock Center | RRID:BDSC_38424 | |
| Genetic reagent (D. melanogaster) | UAS-Snr1RNAi1 | VDRC | 108599 | RNAi1 |
| Genetic reagent (D. melanogaster) | UAS-Snr1RNAi2 | VDRC | 32372 | RNAi2 |
| Genetic reagent (D. melanogaster) | UAS-Gapdh1RNAi | VDRC | 100596 | |
| Genetic reagent (D. melanogaster) | UAS-Pglym78 | VDRC | 106818 | |
| Genetic reagent (D. melanogaster) | UAS-Cyt-c1RNAi1 | VDRC | 9180 | RNAi1 |
| Genetic reagent (D. melanogaster) | UAS-Cyt-c1RNAi2 | VDRC | 109809 | RNAi2 |
| Genetic reagent (D. melanogaster) | UAS-Cyt-c-pRNAi1 | VDRC | 33019 | RNAi1 |
| Genetic reagent (D. melanogaster) | UAS-Cyt-c-pRNAi2 | VDRC | 106759 | RNAi2 |
| Genetic reagent (D. melanogaster) | w, tub-GAL4, UAS-nlsGFP::6xmyc::NLS, hsFLP122; FRT82B, tubP-GAL80/TM6B | Cédric Maurange (Narbonne-Reveau et al., 2016) |
MARCM line | |
| Genetic reagent (D. melanogaster) | FRT82B snr16C hdac36C | Bloomington Drosophila Stock Center (Koe et al., 2014) |
RRID:BDSC_34494 | |
| Genetic reagent (D. melanogaster) | Imp-GFP | Florence Besse | protein trap line | |
| Genetic reagent (D. melanogaster) | poxn-Gal4 | Bloomington Drosophila Stock Center (Boll and Noll, 2002) |
RRID:BDSC_ 66685 | |
| Genetic reagent (D. melanogaster) | ase-Gal80, wor-GAL4, UAS-dcr2 | Juergen Knoblich (Eroglu et al., 2014) |
||
| Genetic reagent (D. melanogaster) | nab-GAL4 | Kyoto DGRC | 6190 | |
| Genetic reagent (D. melanogaster) |
UAS-FLP, Ubi-p63EFRTstopFRTnEGFP | Bloomington Drosophila Stock Center (Evans et al., 2009) |
RRID:BDSC_28282 | G-trace |
| Genetic reagent (D. melanogaster) | hs-mFLP5 | Bloomington Drosophila Stock Center (Hadjieconomou et al., 2011) |
RRID:BDSC_35534 | Flipase for Flybow |
| Antibody | chicken polyclonal anti-GFP | Aves Labs #GFP-1020 |
RRID:AB_10000240 | 1:1000 |
| Antibody | rabbit polyclonal anti-RFP | Rockland #600-401-379 |
RRID:AB_2209751 | 1:500 |
| Antibody | rat monoclonal anti-RFP | Chromotek #5F8 | RRID:AB_2336064 | 1:500 |
| Antibody | mouse monoclonal anti-Miranda | Alex Gould | 1:50 | |
| Antibody | rabbit polyclonal anti-PH3 | Millipore #06–570 | RRID:AB_310177 | 1:500 |
| Antibody | rat monoclonal anti-PH3 | Abcam #AB10543 | RRID:AB_2295065 | 1:500 |
| Antibody | rat monoclonal anti-Elav | DSHB | #9F8A9 | 1:50 |
| Antibody | rabbit polyclonal anti-cleaved Dcp-1 | Cell Signaling #9578 | RRID:AB_2721060 | 1:500 |
| Antibody | rat polyclonal anti-Chinmo | Nick Sokol | 1:500 | |
| Antibody | rabbit polyclonal anti-Imp | Paul Macdonald | 1:500 | |
| Antibody | guinea pig polyclonal anti-Syp | Ilan Davis | 1:500 | |
| Antibody | Rabbit polyclonal anti-Syp | Ilan Davis | 1/200 | |
| Antibody | rabbit polyclonal anti-E93 | Daniel J. McKay | 1/2500 | |
| Antibody | guinea-pig polyclonal antibody | Bill McGinnis | 1/200 | |
| Chemical compound, drug | Dapi | Vector Laboratories Cat# H-1400 | RRID:AB_2336787 | |
| Recombinant DNA reagent | Chinmo cDNA clone | DGRC, EST Collection | #RE59755 | |
| Sequence-based reagent | Chin-5'UTR/pBS_Forward | This paper | PCR primers | Described in Affinity pull-down assays section in the Materials and methods |
| Sequenced-based reagent | Chin-5'UTR/pBS_Reverse | This paper | PCR primers | Described in Affinity pull-down assays section in the Materials andmethods |
| Sequenced-based reagent | Chin-3'UTR/pBS_Forward | This paper | PCR primers | Described in Affinity pull-down assays section in the Materials and methods |
| Sequenced-based reagent | Chin-3'UTR/pBS_Reverse | This paper | PCR primers | Described in Affinity pull-down assays section in the Materials andmethods |
| Software, algorithm | Seurat and Monocle codes used for single-cell RNA-seq data analysis | This paper | https://github.com/cedricmaurange/Genovese-et-al.-2019 | See the Single-cell mRNA sequencing and analysis section in the Materials and methods |
| Software, algorithm | Code used for numerical model | This paper | http://dx.doi.org/10.17632/j2j9gmyb6m.1 | See the Numerical model section in the Materials and methods |