Figure 3.

Nr2f6-Deficient B Cells Do Not Contribute to the GC Phenotype of Nr2f6^−/− Mice

(A) Experimental scheme showing CD4 depletion of Nr2f6^+/+ or Nr2f6^−/− host mice followed by Nr2f6^+/+ OT-II transfer.

(B) Splenocyte stain from wild-type control mouse, with no depletion or OT-II transfer, harvested 7 days after OVA-alum immunization and stained for CD4, Vα2, and Vβ5.

(C) Representative plots of Vα2⁺ Vβ5⁺ CD4⁺ T cells 7 days after immunization and 8 days after OT-II transfer. Combined frequency and total cell counts are displayed in the right two panels.

(D) Vα2⁻ Vβ5⁻ CD4⁺ CXCR5⁺ PD-1⁺ T cell (host) frequency and total cell counts are displayed with representative flow cytometry plots.

(E) Frequency of the Tfh cell population from transferred OT-II cells (CD4⁺ Vα2⁺ Vβ5⁺) and total cell counts are displayed with representative flow cytometry plots.

(F and G) Host germinal center B cell (F) and plasma cell frequency (G), as well as total cell counts, are displayed.

Data shown are from two independent experiments with n ≥ 6. The middle bar represents the dataset average. Error bars represent SD, and an asterisk indicates statistically significant differences calculated using Student’s t test. A p value of <0.05 was considered statistically significant. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.