REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD4 (RM4-5)	BD Biosciences	Cat#560782; RRID:AB_1937315
PD-1 (J43)	Thermo Fisher	Cat#11-9985-82; RRID:AB_465472
Ki67 (SolA15)	Thermo Fisher	Cat#12-5698-80; RRID:AB_11149672
CD45 (30-F11)	BD Biosciences	Cat#561487; RRID:AB_561487
CD45.1 (A20)	Biolegend	Cat#110716; RRID:AB_10660302
CD45.1 (A20)	Thermo Fisher	Cat#25-0453-81; RRID:AB_469628
CD45.2 (clone 104)	BD Biosciences	Cat#562130; RRID:AB_10896649
FoxP3 (FJK-15 s)	Thermo Fisher	Cat#11-5773-82; RRID:AB_465243
CXCR5 (2G8)	BD Biosciences	Cat#551960; RRID:AB_394301
CD19 (ID3)	Thermo Fisher	Cat#25-0193-82; RRID:AB_657663
CD19 (ID3)	BD Biosciences	Cat#561113; RRID:AB_10563071
B220 (RA3-6B2)	Thermo Fisher	Cat#12-0452-82; RRID:AB_465671
B220 (RA3-6B2)	Biolegend	Cat#103236; RRID:AB_893354
Fas (Jo2)	BD Biosciences	Cat#563647; RRID:AB_2738346
GL7 (GL7)	BD Biosciences	Cat#553666; RRID:AB_394981
CD86 (GL1)	Biolegend	Cat#105014; RRID:AB_439783
CXCR4 (L276F12)	Biolegend	Cat#146509; RRID:AB_2562786
CD138 (281-2)	Biolegend	Cat#142503; RRID:AB_10915989
CD138 (281-2)	BD Biosciences	Cat#562610; RRID:AB_11153126
IgD (11-26c.2a)	Biolegend	Cat#405711; RRID:AB_1937245
IgG1 (A85-1)	BD Biosciences	Cat#550331; RRID:AB_2296342
IgM (II/41)	Thermo Fisher	Cat#25-5790; RRID:AB_469655
PD-L2/CD273 (122)	Thermo Fisher	Cat#11-9972-82; RRID:AB_465462
CD80 (16-10A1)	Biolegend	Cat#104713; RRID:AB_1240733
Tbet (4B10)	Thermo Fisher	Cat#50-5825-80; RRID:AB_10597459
GATA3 (16E10A23)	Biolegend	Cat#653809; RRID:AB_2563216
BCL6 (K112-91)	BD Biosciences	Cat#562198; RRID:AB_11153136
IL-17a (TC11-18H10)	BD Biosciences	Cat#560220; RRID:AB_1645194
CD38 (90)	Biolegend	Cat#102727 RRID:AB_2616967
Gr-1 (RB6-8C5)	Biolegend	Cat#108437 RRID:AB_2562214
F4/80 (BM8)	Biolegend	Cat#123135 RRID:AB_2562622
IL-21R (2A9)	Bioxcell	Cat#BE0258; RRID:AB_2687737
Chemicals, Peptides, and Recombinant Proteins
Ovalbumin (OVA)	Hyglos	Cat#321001
NP-chicken gamma globulin (NP-CGG) (Ratio 20)	Biosearch Technologies	Cat#N-5055C-1
NP-Ovalbumin (NP-OVA) (Ratio 20 or 17)	Biosearch Technologies	Cat# N-5051-10-BS
Aluminum potassium sulfate dodecahydrate (Alum)	Sigma	Cat#31242-500G-D
Strepavidin APC	BD Biosciences	Cat#554067
Strepavidin APC-Cy7	Biolegend	Cat#405208
NP-PE (Ratio 28)	Biosearch Technologies	Cat#N-5070-1
7-amino-actinomycin D (7-AAD)	Biolegend	Cat#420403
AnnexinV-PE	Biolegend	Cat#640908
Sodium Hydroxide	Fisher	Cat# S318-1
Sheep Red Blood Cells (SRBCs)	Labor Dr. Merck, Ochsenhausen	Cat#E-400
Critical Commercial Assays
Thermo Fisher FoxP3 Transcription Factor Staining Buffer Set	Fisher	Cat#00-5523-00
RNA Isolation Kit	QIAGEN	Cat#GR8RNA
Omniscript RT Kit 200	QIAGEN	Cat#205113
qRT-PCR Kit (Rox)	Bio & SELL	Cat#BS76.520.5000
Chromatrap® pro G ChIP spin column kit	Direct 7Bioscience	Cat#500190
Bio-Plex Pro Mouse Cytokine IL-21	Biorad	Cat#171GA003M
Experimental Models: Cell Lines
Jurkat T cells	ATCC	ATCC-TIB152
Experimental Models: Organisms/Strains
Nr2f6-deficient mice (C57BL/6)	Hermann-Kleiter et al., 2008	N/A
Wild-type, C57BL/6N	Charles River	Strain Code#027
Nr2f6+/+ OT-II, C57BL/6-Tg(TcraTcrb) 425Cbn/Crl	Charles River	Strain Code#643
Nr2f6−/− OT-II	In House	N/A
B6.SJL-Ptprca Pepcb/BoyJ (CD45.1)	Jackson	Stock# 002014; RRID:IMSR_JAX:002014
B6.SJL x C57BL/6 (CD45.1/CD45.2)	In House	N/A
Oligonucleotides
See Table S1 for EMSA Oligonucleotide sequences	This paper	N/A
CNS +23 F-ACTTCCACTGGTCAGGGTCT	Synthesized by Eurofins	N/A
CNS +23 R-AGCAATGGTCAATTGTTGTGGG	Synthesized by Eurofins	N/A
CNS −36 F-CTCCAGTGACCCCATTCTCC	Synthesized by Eurofins	N/A
CNS −36 R-TTGCTCTCGCCAAGTGGAAA	Synthesized by Eurofins	N/A
Prom −1- −2.2kb F-CAGCAGCGTCCCTTACAGAA	Synthesized by Eurofins	N/A
Prom −1- −2.2kb R-CTTCTGCAGGAGATGAGCATT	Synthesized by Eurofins	N/A
qRT-PCR primers Il21	Applied Biosystem	Cat#Mm00517640_m1
qRT-PCR primers Il4	Applied Biosystem	Cat#Mm00445259_m1
qRT-PCR primers Il2	Applied Biosystem	Cat#Mm00434256_m1
Recombinant DNA
Nr2f6-pEFNeos construct	Hermann-Kleiter et al., 2008	N/A
Software and Algorithms
FlowJo 10	TreeStar	RRID:SCR_008520
Fiji (ImageJ)	N/A	http://fiji.sc; RRID:SCR_002285
Prism 7	Graphpad	https://www.graphpad.com/; RRID:SCR_002798
R	N/A	https://www.r-project.org/