Figure 3.
Identification of the causative deletion using aCGH. (a) Bulked segregant analysis was used to map the short trichome phenotype to a 7.28 Mb region on chromosome 6, which is shaded in blue. A single deletion was identified in the mapping interval using aCGH. The cluster of probes with log2 ratios below 0.0 indicate an absence of DNA in the mutant (R59C46) compared to the wild-type (M92-220). (b) Primers were designed to span the deletion and amplicons were sequenced to identify the exact breakpoint edges. The deleted interval is shaded in red. The deletion encompassed six gene models, indicated in the diagram by arrows. The candidate gene GmCPR5 (Glyma.06g145800) is indicated by a solid black arrow.