Fig. 2.

Detection of LINC complex components at meiotic telomere attachment sites. a SUN1, KASH5, and SYCP3 triple localization on murine testis cell spreadings imaged with structured-illumination microscopy. SUN1 and KASH5 localize to the two ends of the SYCP3 signal. Inset: magnification of one terminal SYCP3 signal revealing that SUN1 and KASH5 signals are densely packed, with the SUN1 signal likely residing in between KASH5 and SYCP3. b Immunogold localization of SUN1 on epoxy-embedded frozen sections. Arrowheads point to gold particles localizing to the perinuclear domain of the filaments that emanate from the inner nuclear envelope at attachment sites. c Immunogold localization of the KASH5 N-terminus. Colloidal gold bound to the end of the cytoplasmic filaments is indicated by arrowheads. LE: lateral element, CE: central element, AP: attachment plate, Ch: Chromatin, NE: nuclear envelope. Scale bars: (a): 2 µm, (b, c): 100 nm