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. 2019 Oct 14;9:14731. doi: 10.1038/s41598-019-51184-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The dependence of TPP⁺ absorption by yeast cells over time after PEF treatment. The y axis represents the relative TPP⁺ amount accumulated in the yeast cells; the x axis represents the TPP⁺ injection time after PEF treatment. The dashed line represents the full recovery of the TPP⁺ absorption level by the yeast cells (PEF = 0) after 3 minutes of incubation. The solid curves were obtained by using Eq. (10), adj. R² values are 0.854 (rectangles), 0.582 (triangles) and 0.341 (circles).