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. 2019 Oct 14;10(10):778. doi: 10.1038/s41419-019-2021-3

Fig. 4. Inhibition of cathepsin B alleviated KD-treated EC pyroptosis.

Fig. 4

HUVECs were pretreated with a cathepsin B inhibitor (CA074-Me, 25 μM) for 30 min, and then ECs were co-cultured with pooled sera-treated THP1 for 24 h. a Endothelial cells were stained with Magic Red Cathepsin B detection reagent to examine the activation of cathepsin B. Magnification: ×200, Scale bar = 100 μm. b Effects of CA074-Me treatment on the expression of pyroptosis-associated proteins were assessed in the treated endothelial cells. c–i Quantitative analysis of pyroptosis-related protein expression, and GAPDH was used as an internal control. Data were exhibited as mean ± SD (n = 3). j–m Caspase-1 expression, TUNEL staining, LDH release, and Hoechst 33342/PI double staining were conducted as described in Fig. 2c–f. Magnification: ×200, Scale bar = 100 μm for (j) and (k). Magnification: ×100, Scale bar = 500 μm for (m). Significance: *P < 0.05, and **P < 0.01 indicate significant difference between KD group and HC group. #P < 0.05 indicates significant difference between KD groups with and without CA074-Me addition