Figure 2.
Intracellular viability of L. amazonensis-infected THP-1 cells treated with 17-DMAG. THP-1 cells (5 × 105 per well) were plated in RPMI medium with PMA (100 nM) to promote differentiation into macrophages, then infected with L. amazonensis promastigotes (10:1) for 6 h at 35 °C. After washing to remove non-internalized parasites, cells were treated with 17-DMAG at concentrations of 6.25 nM, 12.5 nM, 25 nM, 50 nM, 100 nM, 150 nM, 200 nM, 250 nM, 300 nM and 400 nM (five replicates per concentration) for 72 h at 35 °C. Cells were washed, Schneider’s complete medium was added and, after four days at 24 °C, parasites were counted in a Neubauer chamber. Mann Whitney test **p < 0.01.