Fig. 4. PGKO mice resisted weight and adiposity gained from long-term HFD.

Left-hand figures are male data, right-hand figures are female data. Terminal body weight was measured in male (a) and female (c) mice after 8 and 10 months of HFD, respectively. Fat pad (i.e., epididymal white adipose tissue, EWAT) was dissected from euthanized mice and weighed for males (b) and females (d). Body weight was assessed at the time of indirect calorimetry and 5-months post calorimetry (see Fig. 3) for males (e) and females (g). The difference between these two time points (i.e., weight gain) was calculated for males (f) and females (h). Data shown are average ± standard error. Statistics were calculated with student’s t-test (n = 7, 8 male; n = 9, 11 female). *p < 0.05 was considered significant; **p < 0.01, additional p-values are indicated for clarity