Fig. 2. CBD induces ultrastructural alterations in Jurkat cells.

a, b Representative TEM images of untreated Jurkat cells (a) and Jurkat cells, fixed after 2 h of incubation with CBD (30 μM, b). Scale bars are indicated. c, d High-resolution TEM images of control (c) and CBD-treated cells (d). Scale bar: 2 μm. Cell components are indicated as following: nucleus (N), Golgi apparatus (GA), mitochondria (M), endoplasmic reticulum (ER), and autophagic vacuoles (AV). Note autophagic bodies (ABd) in b, panel a; inclusion of condensed chromatin in one of ABds is indicated by arrow. An electron dense lysosome in the neighborhood of an autophagic vacuole is indicated by arrow in c