Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 14;9:14762. doi: 10.1038/s41598-019-51381-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Sustained HP inhibits cell proliferation and induces ciliogenesis in SHED. (a) Cell proliferation analysis by a cell counting method in mDP cells (a) and SHED (b). The cells were cultured with or without the HP by the medium height of 5 cm (H: 5cm) for 24, 48 and 72 hrs. (c,d) Immunostaining of acetylated α-tubulin in mDP cells (c), Scale bar: 50 μm; and SHED (d), Scale bar: 25 μm. The cells were cultured with or without the HP by the medium height of 5 cm (H: 5 cm) for 6 hrs, and then immunostaining was perfomed for acetylated α-tubulin. The acetylated α-tubulin-positive cells were counted in twenty randomly selected fields of view under an inverted microscope (20X magnification). The bar graph shows the percentage of α-tubulin-positive cells of total nuclear-stained cells. Red, acetylated α-tubulin; blue, DAPI. The data presented in (a,b) is a representative of three independent experiments showed similar results. (c,d) Represent the mean (±standard deviation, SD) of three independent experiments, and each performed in triplicate. Statistical analysis was performed using analysis of variance (**p < 0.01).