Table 1.
Methylated motifs in C. thermocellum ATCC27405
| Motif | Type | % Modified | # Motifs in the genome | Predicted methyltransferase |
|---|---|---|---|---|
| GATC | m6A | 74 | 8234 | Cthe_2470 and 1511 |
| CNCANNNNNNTTC | m6A | 57.1 | 1775 | Cthe_1144–1145 |
| GTCAT | m6A | 49.6 | 6945 | Cthe_0519 |
| GCWGC | m5C | 100 | 6283 | Cthe_1749 |
| GGCC | m5C | 100 | 12,192 | Cthe_2321 |
Methylated bases are in bold. In cases where T or G are bold, the methylation is on the A or C of the complementary strand, respectively. % m6A motifs were detected by PacBio SMRT sequencing, and m5C motifs were detected by WGBS. Modified is the percentage of these motifs in the genome that were detected as methylated. # of motifs in the genome is the number of times each motif appears in the genome. The “Predicted Methyltransferase” is the most likely C. thermocellum gene responsible for each methylation (N any base, W A or T)