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. 2019 Oct 8;10:1159. doi: 10.3389/fphar.2019.01159

Figure 7.

Figure 7

Synergistic action of combining phenformin with THP in vivo. (A) Images of THP combined with phenformin treated animals in mouse orthotopic implantation model. (B) Kaplan–Meier survival analysis of four groups. Death of mice was checked daily, and cumulative survival rate was plotted against the time course. All living mice were killed at day 28. (C) The weight of mice was measured daily. All bladder tissues were collected and fixed. (D) The images of the explanted bladder tumors. (E) Weights of mouse bladders including those that died before the end of experiment were measured. Shown are means and SD. **/***P < 0.01, *P < 00.05. (F) Western blot analysis of pAkt, pErk, and p-mTOR protein expression in bladder tissues from mice of five groups. (G) Relative levels of phosphorylated Akt, Erk, and mTOR are shown as means ± SD, ***P < 0.001, **P < 0.01, *P < 0.05. (H) Immunohistochemistry was used to detect the expression of pAkt, pErk, and p-mTOR of bladder tissues from mice of five groups.