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. 2019 Oct 14;9:14748. doi: 10.1038/s41598-019-51319-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sperm quality of split semen samples (n = 12 boars) assigned to four groups: (1) BTS extender with antibiotics, storage at 17 °C (17 °C, BTS), (2) BTS extender with antibiotics (AB), storage at 5 °C (5 °C, BTS), (3) AndroStar Premium with AB, storage at 17 C° (17 °C, APrem), (4) APrem without AB, storage at 5 C° (5 °C, APrem). (A) Total motile spermatozoa (%; means ± SEM) after 24, 72, and 144 h of storage. (B) Plasma membrane and acrosome intact (PI-negative and FITC-PNA negative) spermatozoa (%; means ± SEM) after 72 h of storage. (C) DNA fragmentation index (DFI %; means ± SEM) after 72 h of storage. *Values of 5 °C, BTS differ from all other semen groups at a given storage time (p < 0.05).