Skip to main content
. 2019 Sep 2;38(20):e101430. doi: 10.15252/embj.2018101430

Figure EV4. Cyclin F controls transcription of DNA repair via E2F7/8.

Figure EV4

  1. Venn diagrams of differentially expressed transcripts in RNA‐sequencing analysis of nocodazole‐arrested HeLa cells depleted of cyclin F and/or E2F7/8 as indicated.
  2. KEGG pathway analysis of genes upregulated by cyclin F knockdown and rescued by additional E2F7/8 depletion. Bars represent −log P‐values, such that larger values mean stronger statistical significance. The cutoff P‐value 0.05 is shown as a red dotted line.
  3. qPCR assay showing the expression of atypical E2F target genes that are involved in DNA damage and repair. RPE cells were transfected for 48 h with siRNA as indicated. Sixteen hours before harvesting, cells were treated with nocodazole. Data represent averages ± SEM (n = 3); *P < 0.05 or **P < 0.01 (Student's t‐test). n.s.: not significant.
  4. qPCR showing the RNA expression of PLK1 and CCNB1. HeLa cells were transfected for 48 h with siRNAs as indicated. Cells were incubated with nocodazole 16 h before harvesting. Data represent averages ± SEM (n = 3); **P < 0.01 (Student's t‐test). n.s.: not significant.
  5. Phosphorylated MPM2 staining in flow cytometry demonstrated that mitotic activity was regulated in a cyclin F‐dependent manner.