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. 2019 Sep 2;38(20):e101430. doi: 10.15252/embj.2018101430

Figure 6. Cyclin F regulation of DNA replication and DNA repair genes is dependent on E2F7/8.

Figure 6

  1. Heatmap showing differentially expressed genes after cyclin F knockdown, and rescued by additional E2F7/8 depletion. Highlighted genes are all involved in DNA repair. HeLa cells were arrested with nocodazole for 16 h prior to harvesting to minimize bias from potential differences in cell cycle progression between the different conditions.
  2. KEGG pathway analysis of genes downregulated by cyclin F knockdown, and rescued by additional E2F7/8 depletion. Bars represent −log P‐values, such that larger values mean stronger statistical significance. The cutoff P‐value 0.05 is shown as a red dotted line.
  3. qPCR showing the RNA expression of atypical E2F target genes that are involved in DNA replication or DNA repair. HeLa cells were transfected for 48 h with siRNAs as indicated. Cells were incubated with nocodazole for 16 h before harvesting. Data represent averages ± SEM (n = 3); *P < 0.05 or **P < 0.01 (Student's t‐test). n.s.: not significant.
  4. Immunoblotting showing the protein levels of Chk1 and RAD51 in the indicated siRNA conditions. HeLa cells were treated with nocodazole for 16 h prior to harvesting.

Source data are available online for this figure.