Figure EV1. Cyclin F loss promotes cell death after checkpoint inhibition (related to Fig 1).

1. Cell cycle profile of HeLa and CCNF K/O detected by FACS using DAPI.
2. Cell cycle distribution of untreated HeLa and CCNF K/O cells using combined phospho‐histone H3 Serine 10 and EdU staining.
3. Cell survival of RPE cells transfected with non‐targeting siRNA siNC (negative control) or siCyc F after treatment with Chk1i (LY2603618) at indicated concentrations compared to DMSO‐treated controls (NT).
4. Cell survival measured using resazurin and compared to controls treated with DMSO (expressed as relative proliferation %). Cells were treated with ATR inhibitors at the indicated concentrations.
5. Cell survival of U‐2‐OS cells transfected with non‐targeting siRNA siNC (negative control) or siCyc F after treatment with Chk1i (LY2603618) at indicated concentrations compared to DMSO‐treated controls (NT).

Data information: Data are presented as mean ± SD, with at least three independent experiments. P‐values (*P < 0.05, **P < 0.005) were calculated by paired and two‐tailed t‐test.