Figure 10. Effect of torin-1 at 10 µM on lipolysis.

Adipocytes were pre-incubated without (blue) or with 10 µM torin-1 (magenta) for 30 min, when the indicated concentration of insulin was added and cells incubated for 15 min before incubation with 10 nM isoproterenol (iso) for another 10 min. Cells were then separated from medium and the phosphorylation of PKB at Ser⁴⁷³ (n = 4) (a) and Thr³⁰⁸ (n = 5) (b), and cAMP (n = 3) (d) was determined in the cells, and glycerol (n = 8) (c) determined in the medium. Indicated is P-value for the effect of torin-1 in isoproterenol-stimulated cells (c). Representative immunoblots of one experiment are shown (a and b).