Figure 6. Effect of wortmannin on lipolysis.

Adipocytes were pre-incubated without (blue) or with 0.5 µM wortmannin for 30 min (green), when the indicated concentration of insulin was added and cells incubated for 15 min before incubation with 10 nM isoproterenol (iso) for another 10 min. Cells were then separated from medium and glycerol released to the medium was determined (n = 5) (a), and cAMP (n = 9) (b), phosphorylation of PKB at Ser⁴⁷³ (n = 6) (c) and Thr³⁰⁸ (n = 6) (d) were determined in the cells. Indicated are P-values for the effect of wortmannin in isoproterenol-stimulated cells (a and b). Representative immunoblots of one experiment are shown (c and d).