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Validation of TALe clones through PCR, restriction enzyme digestions. (A) Schematic of selective isolation of SphI fragments TALe genes from genomic DNA of PXO61. (B) Validation of TALe clones through PCR with primers P‐F1 and P‐R1 of individual clones as indicated above lanes of upper gel image, digestion by MscI which cuts each of central repeats (the DNA band patterns resulted from partial digestion) and digestion by SphI.
